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Ana slipped to a 6-3 6-4 loss against world No.14 Marion Bartoli in the second round of the Bank of the West Classic in Stanford. It was the first loss for the 22-year-old in four matches against the Frenchwoman.
The defending champion served at a consistently high level, while Ana didn’t manage to win more than 37 per cent of the points on her second serve.
Ana broke to lead early in the second set, but couldn't sustain her advantage.
"She was hitting the ball faster than I thought she would," said Ana, who had previously lost just one set against the Frenchwoman.
"It was very hard for me to read her serve. I always felt I was a little bit too late.
"But I had some chances. Overall there are a lot of positive things from this match. I just have to move forward."
Ana will now head to San Diego, where she enters the Mercury Insurance Open, which starts on Monday.