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Ana has been drawn to face American world No.44 Madison Keys in the first round of the Mutua Madrid Open, which begins tomorrow.
All of the world's top 20-ranked players are present, with the exception of Victoria Azarenka. In her absence, Ana is seeded 11th.
If she reaches the second round Ana will take on either countrywoman Bojana Jovanovski or Italy's Karin Knapp. Sixth seed Jelena Jankovic is a possible third round opponent, in what would be the pair's 13th WTA meeting.
In the quarter-finals Ana could face fourth seed Simona Halep, while world No.1 and two-time defending champion Serena Williams is a possible opponent in the last four.
The leading seeds in the bottom half of the draw, whom Ana cannot face until the final, are No.2 Na Li, No.3 Agnieszka Radwanska, No.7 Angelique Kerber and No.8 Maria Sharapova.
Ana reached the semi-finals of this event last season.
Click here for the full draw.