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Ana takes on German's Mona Barthel on Wednesday at the world No.35's home event, the Porsche Tennis Grand Prix in Stuttgart.
Ana has never before played the 21-year-old, who despite her ranking required a wild card into the $740,000 event, which takes place on indoor clay.
Including qualifying, Barthel has won a whopping 24 matches already this year and she sits at 17th in the Race to the WTA Championships Singles Standings. Most notably, she beat former world No.1 Jelena Jankovic 6-0, 6-3 in MIami last month.
"Of all the tournaments on the Tour, this is one where there really is no easy match because the field is just so strong," said Ana.
"Barthel has had some great results this year, she's playing in front of her home crowd and I'm expecting a very tough challenge."
The match is scheduled to begin at 2pm local time (8am Eastern Time). Click here for live point-by-point scoring.